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Identification of Kinase-substrate Pairs Using High Throughput Screening

DOI :

10.3791/53152-v

August 29th, 2015

August 29th, 2015

7,949 Views

1Children's Hospital of Eastern Ontario Research Institute, 2Sunnybrook Research Institute, University of Toronto, 3Department of Biochemistry, University of Toronto

Protein phosphorylation is a central feature of how cells interpret and respond to information in their extracellular milieu. Here, we present a high throughput screening protocol using kinases purified from mammalian cells to rapidly identify kinases that phosphorylate a substrate(s) of interest.

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