JoVE Logo
Faculty Resource Center

Sign In

Assaying the Kinase Activity of LRRK2 in vitro

DOI :

10.3791/3495-v

6:09 min

January 18th, 2012

January 18th, 2012

22,070 Views

1Department of Molecular Neuroscience, UCL Institute of Neurology

Leucine Rich Repeat Kinase 2 is a large multidomain kinase, mutations in which are the most common genetic cause of Parkinson's disease. Analysis of the kinase activity of this protein has proven to be a crucial tool in understanding the biology and dysfunction of this protein. In this paper, in vitro assaying of the kinase activity of LRRK2 and a selection of its mutants is described, providing an experimental system to examine phosphorylation of putative substrates and potential dysfunction of LRRK2 in disease.

Tags

Assaying

-- Views

Related Videos

article

Assaying the Ability of Diffusible Signaling Molecules to Reorient Embryonic Spinal Commissural Axons

article

In vitro Reconstitution of the Active T. castaneum Telomerase

article

Monitoring Kinase and Phosphatase Activities Through the Cell Cycle by Ratiometric FRET

article

Direct Detection of the Acetate-forming Activity of the Enzyme Acetate Kinase

article

Rapid Generation of Amyloid from Native Proteins In vitro

article

Assaying Proteasomal Degradation in a Cell-free System in Plants

article

Functional Reconstitution and Channel Activity Measurements of Purified Wildtype and Mutant CFTR Protein

article

Measurement of Larval Activity in the Drosophila Activity Monitor

article

In Vitro Assay to Measure Phosphatidylethanolamine Methyltransferase Activity

article

Measuring In Vitro ATPase Activity for Enzymatic Characterization

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved