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Studying the Stoichiometry of Epidermal Growth Factor Receptor in Intact Cells using Correlative Microscopy

DOI :

10.3791/53186-v

9:16 min

September 11th, 2015

September 11th, 2015

8,013 Views

1INM-Leibniz Institute for New Materials, 2Department of Physics, University of Saarland

This protocol describes the labeling of epidermal growth factor receptor (EGFR) on COS7 fibroblast cells, and subsequent correlative light- and electron microscopy of whole cells in hydrated state. The label contained fluorescent quantum dots. The protocol can be used to study the stoichiometry of EGFR at the single molecule level.

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Stoichiometry

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