JoVE Journal

Immunology and Infection

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Imaging Neutrophils and Monocytes in Mesenteric Veins by Intravital Microscopy on Anaesthetized Mice in Real Time

We detail a protocol to monitor the behavior of neutrophils and monocytes in mesenteric veins under steady state and inflammatory conditions using intravital confocal microscopy on anaesthetized mice.

Chapters in this video

0:05

Title

1:00

Bone Marrow Single Cell Suspension Preparation

2:38

Neutrophil Enrichment

4:18

Neutrophil Labeling

5:29

In vivo Measurement of Neutrophil and Monocyte Behavior

7:44

Results: Representative Intravital Neutrophil and Monocyte Imaging

8:46

Conclusion

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