March 1st, 2022
•Real-time cell metabolic flux assay measures the oxygen consumption rate and extracellular acidification rate, which corresponds to mitochondrial and glycolytic adenosine triphosphate production, using pH and oxygen sensors. The manuscript explains a method to understand the energy status of osteoblasts and the characterization and interpretation of the cellular bioenergetic status.
Related Videos
Combining QD-FRET and Microfluidics to Monitor DNA Nanocomplex Self-Assembly in Real-Time
Quantitative Real-Time PCR using the Thermo Scientific Solaris qPCR Assay
Stable Isotopic Profiling of Intermediary Metabolic Flux in Developing and Adult Stage Caenorhabditis elegans
Determining Genetic Expression Profiles in C. elegans Using Microarray and Real-time PCR
Sorting of Streptomyces Cell Pellets Using a Complex Object Parametric Analyzer and Sorter
Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance (SPR)
Real Time Analysis of Metabolic Profile in Ex Vivo Mouse Intestinal Crypt Organoid Cultures
Silencing of BRCA2 to Identify Novel BRCA2-regulated Biological Functions in Cultured Human Cells
Real-time Imaging of Plant Cell Surface Dynamics with Variable-angle Epifluorescence Microscopy
Mixed Primary Cultures of Murine Small Intestine Intended for the Study of Gut Hormone Secretion and Live Cell Imaging of Enteroendocrine Cells
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved