JoVE Logo
Faculty Resource Center

Sign In

Identification of Inositol Phosphate or Phosphoinositide Interacting Proteins by Affinity Chromatography Coupled to Western Blot or Mass Spectrometry

DOI :

10.3791/59865-v

July 26th, 2019

July 26th, 2019

8,127 Views

1Institute of Parasitology, McGill University

This protocol focuses on the identification of proteins that bind to inositol phosphates or phosphoinositides. It uses affinity chromatography with biotinylated inositol phosphates or phosphoinositides that are immobilized via streptavidin to agarose or magnetic beads. Inositol phosphate or phosphoinositide binding proteins are identified by Western blotting or mass spectrometry.

-- Views

Related Videos

article

PeptiQuick, a One-Step Incorporation of Membrane Proteins into Biotinylated Peptidiscs for Streamlined Protein Binding Assays

article

A High-Throughput Enzyme-Coupled Activity Assay to Probe Small Molecule Interaction with the dNTPase SAMHD1

article

Author Spotlight: Characterizing DNA G-Quadruplex by Bis-3-Chloropiperidine Based Chemical Mapping

article

Affinity Purification of a Fibrinolytic Enzyme from Sipunculus nudus

article

Author Spotlight: Unveiling Mitochondrial Contact Sites and Architectural Insights

article

Author Spotlight: Peptidome Extraction from Small Extracellular Vesicles Isolated from Bone Marrow-Derived Macrophages

article

Author Spotlight: Advancing Biotherapeutic Mass Calculation by Introducing mAbScale, a Python-Based Desktop Application

article

Author Spotlight: A Novel Method for Comprehensive Cell Component Analysis of Cerebral Blood Clots

article

Author Spotlight: A Bicelle Crystallization Setup for ABC Transporter Membrane Proteins to Advance Drug Development

article

Author Spotlight: An Alternative Approach to Protein Quantification by Bradford Assay Using a Smartphone

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved