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Phospho Flow Cytometry with Fluorescent Cell Barcoding for Single Cell Signaling Analysis and Biomarker Discovery

DOI :

10.3791/58386-v

October 4th, 2018

October 4th, 2018

20,820 Views

1Centre for Molecular Medicine Norway (NCMM), Nordic EMBL Partnership, University of Oslo, 2K. G. Jebsen Centre for B cell malignancies and K. G. Jebsen Centre for Cancer Immunotherapy, University of Oslo

Here, a protocol for medium- to high-throughput analysis of protein phosphorylation events at the cellular level is presented. Phospho flow cytometry is a powerful approach to characterize signaling aberrations, identify and validate biomarkers, and assess pharmacodynamics.

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Phospho Flow Cytometry

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