Measuring G-protein-coupled Receptor Signaling via Radio-labeled GTP Binding
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ā¢
10:13 min
ā¢
June 9th, 2017
DOI :
June 9th, 2017
ā¢Chirag Vasavda*1, Nicholas W. Zaccor*1, Paul C. Scherer1, Charlotte J. Sumner1,2, Solomon H. Snyder1,3,4
1The Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, 2Department of Neurology and neurosurgery, Johns Hopkins University School of Medicine, 3Departments of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, 4Department of Psychiatry and Behavioral Sciences, Johns Hopkins University School of Medicine
Chapters in this video
0:05
Title
0:37
Expression of Recombinant HA-MOR1, Cell Fractionation, and Membrane Collection
3:55
[35S]GTPĪ³S Binding
6:05
Membrane Filtration
6:59
Liquid Scintillation Counting
7:57
Results: Characterization of G-protein-coupled Receptors by Monitoring Radio-labled GTP Binding
9:01
Conclusion
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