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Advanced Imaging of Lung Homing Human Lymphocytes in an Experimental In Vivo Model of Allergic Inflammation Based on Light-sheet Microscopy

DOI :

10.3791/59043-v

April 16th, 2019

April 16th, 2019

7,430 Views

1Department of Medicine 1, University Hospital of Erlangen, 2Department of Medicine 3, University Hospital of Erlangen

The here introduced protocol allows characterization of the lung homing capacity of primary human lymphocytes under in vivo inflammatory conditions. Pulmonary infiltration of adoptively transferred human immune cells in a mouse model of allergic inflammation can be imaged and quantified by light-sheet fluorescence microscopy of chemically cleared lung tissue.

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