Preparation of a Class II Biosafety Cabinet in the Animal Biosafety Level 4 Laboratory
1:33
Example: Plaque Assay
3:23
Waste Disposal and Cleaning of the Instruments and Biosafety Cabinet
5:06
Example: Fixing and Staining of Plaque Assays
6:08
Removing Samples from the BSL-4 Laboratory
7:01
Results: Adherence to Biosafety Level 4 Suit Laboratory Operating Procedures Ensures Safety of Laboratory Staff
8:13
Conclusion
Transcript
The overall goal of this procedure is to demonstrate safe practices when working in a biosafety level 4 suit laboratory using the plaque assay as an example. This procedure demonstrates the modifications necessary to perform a standard plaque assa
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Performing viral assays in a BSL-4 laboratory is more involved compared to work in a BSL-2 laboratory due to required additional safety precautions. Here, we present an overview of practices and procedures used inside a BSL-4 laboratory illustrating proper Class II biosafety cabinet usage, waste management/disposal, and sample removal.