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A Simplified Method for Ultra-Low Density, Long-Term Primary Hippocampal Neuron Culture

DOI :

10.3791/53797-v

March 5th, 2016

March 5th, 2016

15,198 Views

1Department of Basic Medical Sciences, University of Arizona College of Medicine-Phoenix, 2Jiangsu Provincial Center for Disease Control and Prevention

Low density cultures of primary hippocampal neurons usually require glia feeder layer to supply neurotrophic factors and sustain longevity. We describe here a simplified method to culture ultra-low density neurons on glass coverslips in the presence of a high density neuronal feeder layer, which facilitates investigation of specific neuronal-autonomous mechanisms.

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Ultra low Density

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