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Low-Density Primary Hippocampal Neuron Culture

DOI :

10.3791/55000-v

April 18th, 2017

April 18th, 2017

19,875 Views

1Department of Physiology and Pathophysiology, University of Manitoba, 2Kleysen Institute for Advanced Medicine, Health Sciences Centre

This article describes the protocol for culturing low-density primary hippocampal neurons growing on glass coverslips inverted over a glial monolayer. The neuron and glial layers are separated by paraffin wax beads. The neurons grown by this method are suitable for high-resolution optical imaging and functional assays.

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Low density

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