Name: primary culture of hippocampal neurons from p0 newborn rats
Uploaded: 2008-09-29T00:00:00
Description: Watch this Scientific Journal Video about Primary Culture of Hippocampal Neurons from P0 Newborn Rats at JoVE.com

<p class="jove_content">JLN was supported by MH 68347.</p>

<p class="jove_step">Prior to hippocampal isolation</p>
<p class="jove_content">Before beginning the hippocampal isolation, make sure that all the tools are sterile. Spray down the culture hood with 70% ethanol, and place the tools inside the hood. You will need 6- and 10-cm Petri dishes, sterile poly-L coated glass coverslips, pipettors and tips, disposable pipettes, and an electric pipettor. From this point on, remember to use correct sterile technique.</p>
<ol>
<li>Turn on the water bath and make sure that it is heated up to 37&deg;C. </li>
...

<ol>
	<li>Banker, G., Goslin, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> <em style='font-style: italic'>Culturing Nerve Cells, 2nd Edition</em>. , (1998).</li></ol>

<p class="jove_content">This protocol was developed as a modification of the seminal work of Gary Banker and Kim Goslin <sup>1</sup>. This book is an essential resource for anyone interested in culturing cells -- not only the neuron-enriched cultures described in the current protocol.</p>
<p class="jove_content">The three most critical factors in cell culturing are: sterility, speed, the choice of medium used.</p>
<p class="jove_content"><strong>Sterility</strong> - a laminar flow/sterile hood, aseptic conditions, and a sterile incubator are ...

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<td>Invitrogen</td>
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primary culture of hippocampal neurons from p0 newborn rats

The physiological properties of hippocampal neurons are commonly investigated, especially because of the involvement of the hippocampus in learning and memory. Primary hippocampal cell culturing allows neuroscientists to examine the activity and properties of neurons at the individual cell and single synapse level. In this video, we will demonstrate how to isolate and grow primary hippocampal cells from newborn rats. The hippocampus may be isolated from each newborn animal in as short as 2 to 3 minutes, and the cultures can be maintained for up to two weeks. We will also briefly demonstrate how to use these hippocampal neurons for ratiometric calcium imaging. While this protocol describes the process for the hippocampus, with little to no modification, it can be applied to other regions of the brain.

Watch this Scientific Journal Video about Primary Culture of Hippocampal Neurons from P0 Newborn Rats at JoVE.com

Primary Culture of Hippocampal Neurons from P0 Newborn Rats

<p class="jove_content">The dissection and growth of cells from an individual brain area facilitates investigation of cellular and physiological parameters. We describe a method for primary cell culturing that produces neuron-enriched cultures in a serum-free environment.</p>

The physiological properties of hippocampal neurons are commonly investigated, especially because of the involvement of the hippocampus in learning and ...

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