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Fluorescence Time-lapse Imaging of the Complete S. venezuelae Life Cycle Using a Microfluidic Device

DOI :

10.3791/53863-v

11:30 min

February 28th, 2016

February 28th, 2016

13,232 Views

1Department of Molecular Microbiology, John Innes Centre, Norwich Research Park, 2Department of Biology, Lund University

Streptomyces are characterized by a complex life cycle that has been experimentally challenging to study by cell biological means. Here we present a protocol to perform fluorescence time-lapse microscopy of the complete life cycle by growing Streptomyces venezuelae in a microfluidic device.

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Fluorescence Time lapse Imaging

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