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RNA Purification from Intracellularly Grown Listeria monocytogenes in Macrophage Cells

DOI :

10.3791/54044-v

June 4th, 2016

June 4th, 2016

8,914 Views

1The Department of Molecular Microbiology and Biotechnology, The George S. Wise Faculty of Life Sciences, Tel Aviv University

Here we describe a method for bacterial RNA isolation from Listeria monocytogenes bacteria growing inside murine macrophages. This technique can be used with other intracellular pathogens and mammalian host cells.

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