JoVE Logo
Faculty Resource Center

Sign In

RNA Purification from Intracellularly Grown Listeria monocytogenes in Macrophage Cells

DOI :

10.3791/54044-v

June 4th, 2016

June 4th, 2016

8,939 Views

1The Department of Molecular Microbiology and Biotechnology, The George S. Wise Faculty of Life Sciences, Tel Aviv University

Here we describe a method for bacterial RNA isolation from Listeria monocytogenes bacteria growing inside murine macrophages. This technique can be used with other intracellular pathogens and mammalian host cells.

-- Views

Related Videos

article

Following Cell-fate in E. coli After Infection by Phage Lambda

article

Quantitative Imaging of Lineage-specific Toll-like Receptor-mediated Signaling in Monocytes and Dendritic Cells from Small Samples of Human Blood

article

Differentiating Functional Roles of Gene Expression from Immune and Non-immune Cells in Mouse Colitis by Bone Marrow Transplantation

article

piggyBac Transposon System Modification of Primary Human T Cells

article

Clinical Application of Sleeping Beauty and Artificial Antigen Presenting Cells to Genetically Modify T Cells from Peripheral and Umbilical Cord Blood

article

New Tools to Expand Regulatory T Cells from HIV-1-infected Individuals

article

Investigation of Macrophage Polarization Using Bone Marrow Derived Macrophages

article

Culturing Microglia from the Neonatal and Adult Central Nervous System

article

Quantitative In vitro Assay to Measure Neutrophil Adhesion to Activated Primary Human Microvascular Endothelial Cells under Static Conditions

article

Production and Purification of Non Replicative Canine Adenovirus Type 2 Derived Vectors

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved