Epigenetic Conversion as a Safe and Simple Method to Obtain Insulin-secreting Cells from Adult Skin Fibroblasts

6.8K Views

08:45 min

March 18th, 2016

DOI :10.3791/53880-v

March 18th, 2016

Transcript

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Keywords Epigenetic Conversion

Chapters in this video

0:05

Title

0:50

Skin Fibroblast Isolation

1:53

Fibroblast Culture and Plating for Epigenetic Conversion

4:00

Increase Cell Plasticity Using the De-methylating Agent 5-aza-CR

5:31

Pancreatic Differentiation Protocol

6:47

Results: Morphological and Functional Changes During Epigenetic Conversion

8:02

Conclusion

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