Simple Generation of a High Yield Culture of Induced Neurons from Human Adult Skin Fibroblasts
10.2K Views
•
09:07 min
•
February 5th, 2018
DOI :
February 5th, 2018
•1Department of Experimental Medical Science, Wallenberg Neuroscience Center, Division of Neurobiology and Lund Stem Cell Center, Lund University, 2John van Geest Centre for Brain Repair & Department of Neurology, Department of Clinical Neurosciences and Cambridge Stem Cell Institute, University of Cambridge
Chapters in this video
0:04
Title
1:02
Preparation of Skin Fibroblasts for Reprogramming
1:43
Plating for Reprogramming (Day 1)
3:22
Viral Transduction (Day 0)
4:12
Maintenance of the Converting Cells
4:58
FACS
7:33
Results: Generation of Induced Neurons from Adult Human Dermal Fibroblasts
8:15
Conclusion
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