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Enrichment for Chemoresistant Ovarian Cancer Stem Cells from Human Cell Lines

DOI :

10.3791/51891-v

September 10th, 2014

September 10th, 2014

13,269 Views

1Department of Biochemistry and Molecular Biology, Indiana University School of Medicine

Cancer stem cells (CSCs) are implicated in tumor relapse due to chemoresistance. We have optimized a protocol for selection and expansion of CSCs from ovarian cancer cell lines. By treating cells with the chemotherapeutic cisplatin and culturing in a stem cell promoting media we enrich for non-adherent CSC cultures.

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Enrichment

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