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Evaluation of Stem Cell Properties in Human Ovarian Carcinoma Cells Using Multi and Single Cell-based Spheres Assays

DOI :

10.3791/52259-v

January 3rd, 2015

January 3rd, 2015

9,409 Views

1Department of Biomedicine, University Hospital Basel, 2Department of Internal Medicine II, University Hospital Tübingen

In vitro spheres assays are commonly used to identify cancer stem cells. Here we compare single with multi cell-based spheres assays. The more laborious single cell-based assays or methylcellulose supplementation give more accurate results while multi cell-based assays performed in liquid medium can be highly influenced by cell density.

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Keywords Ovarian Carcinoma

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