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In Vitro Disassembly of Influenza A Virus Capsids by Gradient Centrifugation

DOI :

10.3791/53909-v

March 27th, 2016

March 27th, 2016

8,714 Views

1Institute of Biochemistry, ETH Zurich, 2Department of Biochemistry, University of Zurich, 3Institute for Molecular Health Sciences, ETH Zurich

Disassembly of influenza A virus cores during virus entry into host cells is a multistep process. We describe an in vitro method to analyze the early stages of viral uncoating. In this approach, velocity gradient centrifugation is used to biochemically dissect the steps that initiate uncoating under defined conditions.

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In Vitro Disassembly

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