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Preparation of Viral DNA from Nucleocapsids

DOI :

10.3791/3151-v

August 16th, 2011

August 16th, 2011

20,474 Views

1Department of Molecular Biology, Princeton University

We describe the process of isolating high purity herpesvirus nucleocapsid DNA from infected cells. The final DNA captured from solution is of high concentration and purity, making it ideally suited for high-throughput sequencing, high fidelity PCR reactions, and transfections to produce new viral recombinants.

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