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Simple and Robust in vivo and in vitro Approach for Studying Virus Assembly

DOI :

10.3791/3645-v

March 1st, 2012

March 1st, 2012

12,019 Views

1Department of Plant Pathology and Microbiology, University of California, Riverside , 2Department of Bioengineering, University of California, Riverside

A simple, efficient and robust way to synchronize the delivery of multiple viral components to plant cells via Agrobacterium-mediated transient expression is described. This approach is amenable for studying replication, encapsidation followed by in vitro reassembly of non-viral components into genome depleted optical viral ghosts suitable for biomedical applications.

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