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Imaging Local Ca2+ Signals in Cultured Mammalian Cells

DOI :

10.3791/52516-v

March 3rd, 2015

March 3rd, 2015

10,473 Views

1Neurobiology and Behavior, University of California, Irvine, 2Physiology and Biophysics, University of California, Irvine

Here we present techniques for imaging local IP3-mediated Ca2+ events using fluorescence microscopy in intact mammalian cells loaded with Ca2+ indicators together with an algorithm that automates identification and analysis of these events.

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Local Ca2 Signals

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