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A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

DOI :

10.3791/53988-v

11:15 min

May 30th, 2016

May 30th, 2016

23,733 Views

1Department of Chemical Engineering and Biotechnology, University of Cambridge

This article provides an in depth guide for the assembly and operation of a structured illumination microscope operating with total internal reflection fluorescence illumination (TIRF-SIM) to image dynamic biological processes with optical super-resolution in multiple colors.

Tags

Structured Illumination

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