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High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

DOI :

10.3791/60378-v

14:09 min

November 16th, 2019

November 16th, 2019

6,427 Views

1Department of Physics and Technology, UiT The Arctic University of Norway, 2Vascular Biology Research Group, Department of Medical Biology, UiT The Arctic University of Norway

Chip-based super-resolution optical microscopy is a novel approach to fluorescence microscopy and offers advantages in cost effectiveness and throughput. Here, the protocols for chip preparation and imaging are shown for TIRF microscopy and localization-based super-resolution microscopy.

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High Throughput

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