High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

6.8K Views

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14:09 min

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November 16th, 2019

DOI :

10.3791/60378-v

November 16th, 2019

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David André Coucheron¹, Øystein Ivar Helle¹, Cristina Ionica Øie², Jean-Claude Tinguely¹, Balpreet Singh Ahluwalia¹

¹Department of Physics and Technology, UiT The Arctic University of Norway, ²Vascular Biology Research Group, Department of Medical Biology, UiT The Arctic University of Norway

Summary

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High throughput Imaging

Illumination Decoupling

Cleanroom Procedure

PDMS Layer

Cell Seeding

Imaging Possibilities

Fluid Handling

PBS Solution

Chapters in this video

0:01

Title

1:13

Waveguide Cleaning

2:05

Sample Chamber Preparation

2:41

Fluorescent Labelling

4:12

Setup Components

4:56

Waveguide Coupling

7:26

Diffraction Limited Imaging

8:42

dSTORM Imaging

9:35

dSTORM Image Reconstruction

10:42

Results

12:27

Conclusion

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