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Generation of Marked and Markerless Mutants in Model Cyanobacterial Species

DOI :

10.3791/54001-v

May 29th, 2016

May 29th, 2016

11,393 Views

1Department of Biochemistry, University of Cambridge

Introducing multiple genomic alterations into cyanobacteria is an essential tool in the development of strains for industrial and basic research purposes. We describe a system for generating unmarked mutants in the model cyanobacterial species Synechocystis sp. PCC6803 and marked mutants in Synechococcus sp. PCC7002.

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