JoVE Logo
Faculty Resource Center

Sign In

High-Throughput Robotically Assisted Isolation of Temperature-sensitive Lethal Mutants in Chlamydomonas reinhardtii

DOI :

10.3791/54831-v

10:51 min

December 5th, 2016

December 5th, 2016

9,250 Views

1Laboratory of Cell Cycle Genetics, The Rockefeller University, 2Sainsbury Laboratory, University of Cambridge

Temperature-sensitive (ts) lethal mutants are valuable tools to identify and analyze essential functions. Here we describe methods to generate and classify ts lethal mutants in high throughput.

Tags

High throughput

-- Views

Related Videos

article

Using a Fluorescent PCR-capillary Gel Electrophoresis Technique to Genotype CRISPR/Cas9-mediated Knockout Mutants in a High-throughput Format

article

High Fat Diet Feeding and High Throughput Triacylglyceride Assay in Drosophila Melanogaster

article

Generation of Native Chromatin Immunoprecipitation Sequencing Libraries for Nucleosome Density Analysis

article

Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens

article

High-throughput Identification of Synergistic Drug Combinations by the Overlap2 Method

article

Formaldehyde-assisted Isolation of Regulatory Elements to Measure Chromatin Accessibility in Mammalian Cells

article

Gene-targeted Random Mutagenesis to Select Heterochromatin-destabilizing Proteasome Mutants in Fission Yeast

article

High-throughput DNA Extraction and Genotyping of 3dpf Zebrafish Larvae by Fin Clipping

article

High-Throughput DNA Plasmid Multiplexing and Transfection Using Acoustic Nanodispensing Technology (Video) | JoVE

article

Digital PCR-based Competitive Index for High-throughput Analysis of Fitness in Salmonella (Video) | JoVE

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved