October 20th, 2020
•Here, we present a protocol to profile the interplay between host and pathogen during infection by mass spectrometry-based proteomics. This protocol uses label-free quantification to measure changes in protein abundance of both host (e.g., macrophages) and pathogen (e.g., Cryptococcus neoformans) in a single experiment.
Tags
Related Videos
Use of an Optical Trap for Study of Host-Pathogen Interactions for Dynamic Live Cell Imaging
Use of the EpiAirway Model for Characterizing Long-term Host-pathogen Interactions
A Comparative Approach to Characterize the Landscape of Host-Pathogen Protein-Protein Interactions
Detection of Human Leukocyte Antigen Biomarkers in Breast Cancer Utilizing Label-free Biosensor Technology
Electroporation of Functional Bacterial Effectors into Mammalian Cells
Evaluation of the Efficacy And Toxicity of RNAs Targeting HIV-1 Production for Use in Gene or Drug Therapy
A New Method for Qualitative Multi-scale Analysis of Bacterial Biofilms on Filamentous Fungal Colonies Using Confocal and Electron Microscopy
Ex Vivo Infection of Human Lymphoid Tissue and Female Genital Mucosa with Human Immunodeficiency Virus 1 and Histoculture
Label-free Neutrophil Enrichment from Patient-derived Airway Secretion Using Closed-loop Inertial Microfluidics
Label-Free Identification of Lymphocyte Subtypes Using Three-Dimensional Quantitative Phase Imaging and Machine Learning
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved