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Isolation of Giant Lampbrush Chromosomes from Living Oocytes of Frogs and Salamanders

DOI :

10.3791/54103-v

December 5th, 2016

December 5th, 2016

12,487 Views

1Department of Embryology, Carnegie Institution for Science

We present simple techniques for isolating giant transcriptionally active lampbrush chromosomes from living oocytes of frogs and salamanders. We describe how to observe these chromosomes "alive" by phase contrast or differential interference contrast, and how to fix them for fluorescent in situ hybridization or immunofluorescent staining.

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