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Sample Preparation and Analysis of RNASeq-based Gene Expression Data from Zebrafish

DOI :

10.3791/56187-v

October 27th, 2017

October 27th, 2017

10,257 Views

1Division of Endocrinology, Diabetes and Nutrition, Department of Medicine, University of Maryland School of Medicine

This protocol presents an approach for whole transcriptome analysis from zebrafish embryos, larvae, or sorted cells. We include isolation of RNA, pathway analysis of RNASeq data, and qRT-PCR-based validation of gene expression changes.

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