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A Robotic Platform for High-throughput Protoplast Isolation and Transformation

DOI :

10.3791/54300-v

September 27th, 2016

September 27th, 2016

13,646 Views

1Department of Plant Sciences, University of Tennessee, Knoxville, 2Center for Renewable Carbon, University of Tennessee, Knoxville, 3Department of Mechanical, Aerospace, and Biomedical Engineering, University of Tennessee, Knoxville

A high-throughput, automated, tobacco protoplast production and transformation methodology is described. The robotic system enables massively parallel gene expression and discovery in the model BY-2 system that should be translatable to non-model crops.

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