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An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

DOI :

10.3791/57598-v

10:00 min

May 23rd, 2018

May 23rd, 2018

16,802 Views

1Department of Bioengineering and Therapeutic Sciences, California Institute for Quantitative Biosciences, University of California, San Francisco, 2UC Berkeley-UCSF Graduate Program in Bioengineering, University of California, San Francisco, 3Chan Zuckerberg Biohub

Single-cell sequencing reveals genotypic heterogeneity in biological systems, but current technologies lack the throughput necessary for the deep profiling of community composition and function. Here, we describe a microfluidic workflow for sequencing >50,000 single-cell genomes from diverse cell populations.

Tags

Ultrahigh throughput

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