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Methods for Acute and Subacute Murine Hindlimb Ischemia

DOI :

10.3791/54166-v

June 21st, 2016

June 21st, 2016

15,512 Views

1Division of Cardiology, Department of Medicine, Duke University Medical Center

Surgical induction of hindlimb ischemia in the mouse is useful to examine angiogenesis, however this is compromised in certain inbred mouse strains that display marked ischemia-induced tissue necrosis. Methods are described to induce subacute limb ischemia using ameroid constrictors to circumvent this problem through the induction of gradual arterial occlusion.

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