JoVE Logo
Faculty Resource Center

Sign In

Kinetic Measurement and Real Time Visualization of Somatic Reprogramming

DOI :

10.3791/54190-v

July 30th, 2016

July 30th, 2016

6,453 Views

1Life Sciences Solutions, Thermo Fisher Scientific

The protocol presented in this study describes methods for the real-time monitoring of reprogramming progression via the kinetic measurement of positive and negative pluripotent stem cell markers using flow cytometry analysis. The protocol also includes the imaging-based assessment of morphology, and marker or reporter expression during iPSC generation.

-- Views

Related Videos

article

Generation of Retinal Organoids from Healthy and Retinal Disease-Specific Human-Induced Pluripotent Stem Cells

article

Targeted Microinjection and Electroporation of Primate Cerebral Organoids for Genetic Modification

article

Optogenetic Inhibition of Rho1-Mediated Actomyosin Contractility Coupled with Measurement of Epithelial Tension in Drosophila Embryos

article

Nuclei Isolation from Mouse Cardiac Progenitor Cells for Epigenome and Gene Expression Profiling at Single-Cell Resolution

article

Author Spotlight: Advancing Lens Biomechanics Research Through a Novel Protocol for Imaging Complex Interdigitations and Protein Staining

article

Author Spotlight: Effect of Left Atrial Ligation on Avian Embryonic Hearts and HLHS Implications

article

Author Spotlight: Understanding DNA Damage Response in Mammalian Oocytes and Preimplantation Embryos

article

Author Spotlight: Investigating Asymmetric Cell Division Dynamics: A Protocol for Live-Imaging of Drosophila Larval Brain Explants

article

Author Spotlight: Cistrome Analysis in Mouse Muscle Stem Cells

article

Author Spotlight: Optimizing iPSC Differentiation for Efficient Production to Generate Kidney Organoids

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved