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Assessing Functional Metrics of Skeletal Muscle Health in Human Skeletal Muscle Microtissues

DOI :

10.3791/62307-v

February 18th, 2021

February 18th, 2021

3,327 Views

1Institute of Biomedical Engineering, University of Toronto, 2Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, 3Department of Cell and Systems Biology, University of Toronto

This manuscript describes a detailed protocol to produce arrays of 3D human skeletal muscle microtissues and minimally invasive downstream in situ assays of function, including contractile force and calcium handling analyses.

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