JoVE Logo
Faculty Resource Center

Sign In

Assessing Functional Metrics of Skeletal Muscle Health in Human Skeletal Muscle Microtissues

DOI :

10.3791/62307-v

February 18th, 2021

February 18th, 2021

3,543 Views

1Institute of Biomedical Engineering, University of Toronto, 2Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, 3Department of Cell and Systems Biology, University of Toronto

This manuscript describes a detailed protocol to produce arrays of 3D human skeletal muscle microtissues and minimally invasive downstream in situ assays of function, including contractile force and calcium handling analyses.

Tags

Functional Metrics

-- Views

Related Videos

article

Engineering Skeletal Muscle Tissues from Murine Myoblast Progenitor Cells and Application of Electrical Stimulation

article

Transplantation of Induced Pluripotent Stem Cell-derived Mesoangioblast-like Myogenic Progenitors in Mouse Models of Muscle Regeneration

article

Measurement of Maximum Isometric Force Generated by Permeabilized Skeletal Muscle Fibers

article

Engineered Vascularized Muscle Flap

article

Computed Tomography and Optical Imaging of Osteogenesis-angiogenesis Coupling to Assess Integration of Cranial Bone Autografts and Allografts

article

Applications of In Vivo Functional Testing of the Rat Tibialis Anterior for Evaluating Tissue Engineered Skeletal Muscle Repair

article

Cardiac Muscle-cell Based Actuator and Self-stabilizing Biorobot - PART 1

article

Cardiac Muscle Cell-based Actuator and Self-stabilizing Biorobot - Part 2

article

Semiautomated Longitudinal Microcomputed Tomography-based Quantitative Structural Analysis of a Nude Rat Osteoporosis-related Vertebral Fracture Model

article

Melt Electrospinning Writing of Three-dimensional Poly(ε-caprolactone) Scaffolds with Controllable Morphologies for Tissue Engineering Applications

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved