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Isolation and In vitro Culture of Bone Marrow-Derived Macrophages for the Study of NO-Redox Biology

DOI :

10.3791/62834-v

6:34 min

May 31st, 2022

May 31st, 2022

6,019 Views

1BHF Centre of Research Excellence, Division of Cardiovascular Medicine, Radcliffe Department of Medicine, John Radcliffe Hospital, University of Oxford, 2Wellcome Centre for Human Genetics, University of Oxford

This protocol has been established to culture tetrahydrobiopterin (BH4)- and inducible nitric oxide synthase (iNOS)-deficient primary murine macrophages to study NO-redox biology. The study focuses on reducing potential contamination of BH4 and other artifacts found in traditional isolation and culture methods which may confound experimental outcomes and interpretation of results.

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Isolation

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