JoVE Logo
Faculty Resource Center

Sign In

Quantification of Monocyte Chemotactic Activity In Vivo and Characterization of Blood Monocyte Derived Macrophages

DOI :

10.3791/59706-v

9:57 min

August 12th, 2019

August 12th, 2019

9,206 Views

1Department of Internal Medicine, Section on Molecular Medicine, Wake Forest School of Medicine, 2Department of Biochemistry, Wake Forest School of Medicine

Here we present a protocol to quantify the chemotactic activity of blood monocytes in mouse models, to assess the effects of nutritional, pharmacological and genetic interventions on blood monocyte and to characterize the blood monocytes derived macrophages in mouse models using monocyte-chemoattractant protein-1 (MCP-1)-loaded basement membrane-derived gel plugs.

Tags

Keywords Monocyte Chemotactic Activity

-- Views

Related Videos

article

Activation and Measurement of NLRP3 Inflammasome Activity Using IL-1β in Human Monocyte-derived Dendritic Cells

article

Culture of Macrophage Colony-stimulating Factor Differentiated Human Monocyte-derived Macrophages

article

Measuring Granulocyte and Monocyte Phagocytosis and Oxidative Burst Activity in Human Blood

article

Characterization of Human Monocyte-derived Dendritic Cells by Imaging Flow Cytometry: A Comparison between Two Monocyte Isolation Protocols

article

Generation of Human Monocyte-derived Dendritic Cells from Whole Blood

article

Use of a Monocyte Monolayer Assay to Evaluate Fcγ Receptor-mediated Phagocytosis

article

Analysis of Microglia and Monocyte-derived Macrophages from the Central Nervous System by Flow Cytometry

article

Quantifying Human Monocyte Chemotaxis In Vitro and Murine Lymphocyte Trafficking In Vivo

article

Characterization of Human Monocyte Subsets by Whole Blood Flow Cytometry Analysis

article

In Vitro Stimulation and Visualization of Extracellular Trap Release in Differentiated Human Monocyte-derived Macrophages

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved