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Visualization of Inflammatory Caspases Induced Proximity in Human Monocyte-Derived Macrophages

DOI :

10.3791/63162-v

April 6th, 2022

April 6th, 2022

2,412 Views

1Department of Pediatrics, Division of Hematology-Oncology, Department of Molecular and Cellular Biology, Texas Children’s Hospital William T. Shearer Center for Human Immunobiology, Baylor College of Medicine

This protocol describes the workflow to obtain monocytes-derived macrophages (MDM) from human blood samples, a simple method to efficiently introduce inflammatory caspase Bimolecular Fluorescence Complementation (BiFC) reporters into human MDM without compromising cell viability and behavior, and an imaging-based approach to measure inflammatory caspase activation in living cells.

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Keywords Inflammatory Caspases

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