JoVE Logo
Faculty Resource Center

Sign In

In Vitro Permeation of FITC-loaded Ferritins Across a Rat Blood-brain Barrier: a Model to Study the Delivery of Nanoformulated Molecules

DOI :

10.3791/54279-v

August 22nd, 2016

August 22nd, 2016

7,511 Views

1Dipartimento di Scienze Biomediche e Cliniche Luigi Sacco, Università di Milano, 2Dipartimento di Biotecnologie e Bioscienze, Università di Milano-Bicocca

A method to establish an in vitro model of blood-brain barrier based on a co-culture of rat brain microvascular endothelial cells and astrocytes is described and validated. This system proved to be a valid tool to study the effect of nanoformulation on the trans-barrier permeation of fluorescent molecules.

-- Views

Related Videos

article

Alginate Microcapsule as a 3D Platform for Propagation and Differentiation of Human Embryonic Stem Cells (hESC) to Different Lineages

article

Microbial Control and Monitoring Strategies for Cleanroom Environments and Cellular Therapies

article

Quantitative Characterization of Liquid Photosensitive Bioink Properties for Continuous Digital Light Processing Based Printing

article

A Novel Cell Injection Method with Minimum Invasion

article

Soybean Hairy Root Transformation for the Analysis of Gene Function

article

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing

article

Simple Establishment of a Vascularized Osteogenic Bone Marrow Niche Using Pre-Cast Poly(ethylene Glycol) (PEG) Hydrogels in an Imaging Microplate

article

Author Spotlight: Understanding Chronic Lung Diseases Using 3D Printed Phototunable Hydrogels

article

Methods for Electroporation and Transformation Confirmation in Limosilactobacillus reuteri DSM20016

article

Author Spotlight: Advancing Eye Physiology Research via a Multi-Channel Flow Culture for Optimal Tissue Maintenance and Real-Time Assessment

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved