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Endothelial Cell Transcytosis Assay as an In Vitro Model to Evaluate Inner Blood-Retinal Barrier Permeability Video (Video) | JoVE

DOI :

10.3791/64076-v

June 7th, 2022

June 7th, 2022

3,704 Views

1Department of Ophthalmology, Boston Children’s Hospital, Harvard Medical School

This protocol illustrates an in vitro endothelial cell transcytosis assay as a model to evaluate inner blood-retinal barrier permeability by measuring the ability of human retinal microvascular endothelial cells to transport horseradish peroxidase across cells in caveolae-mediated transcellular transport processes.

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