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Visualization of HIV-1 Gag Binding to Giant Unilamellar Vesicle (GUV) Membranes

DOI :

10.3791/54293-v

July 28th, 2016

July 28th, 2016

8,480 Views

1Department of Microbiology and Immunology, University of Michigan Medical School, 2Department of Biophysics, University of Michigan

We illustrate here an in vitro membrane binding assay in which interactions between HIV-1 Gag and lipid membranes are visually analyzed using YFP-tagged Gag synthesized in a wheat germ-based in vitro translation system and GUVs prepared by an electroformation technique.

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