In recent years, encapsulation within cell-sized lipid bilayer vesicles has proven to be a useful method for in vitro reconstitution experiments. The method we present will greatly help with cytoskeleton reconstitution in synthetic cell research.
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This article introduces a simple method for expeditious production of giant unilamellar vesicles with encapsulated cytoskeletal proteins. The method proves to be useful for bottom-up reconstitution of cytoskeletal structures in confinement and cytoskeleton-membrane interactions.