JoVE Logo
Faculty Resource Center

Sign In

Correlative Light- and Electron Microscopy Using Quantum Dot Nanoparticles

DOI :

10.3791/54307-v

August 7th, 2016

August 7th, 2016

9,451 Views

1South Western Sydney Clinical School, Faculty of Medicine, University of New South Wales Australia, 2School of Medicine, Western Sydney University, 3Correlative Microscopy Group, Ingham Institute for Applied Medical Research, 4Electron Microscopy Laboratory, Department of Anatomical Pathology, Sydney South West Pathology Service, New South Wales Health Pathology, 5School of Medical Sciences, Faculty of Medicine, University of New South Wales Australia

A method is described whereby quantum dot (QD) nanoparticles can be used for correlative immunocytochemical studies of epoxy embedded human pathology tissue. We employ commercial antibody fragment conjugated QDs that are visualized by widefield fluorescence light microscopy and transmission electron microscopy.

-- Views

Related Videos

article

Low-Cost Cryo-Light Microscopy Stage Fabrication for Correlated Light/Electron Microscopy

article

Correlative Microscopy for 3D Structural Analysis of Dynamic Interactions

article

Fluorescence-quenching of a Liposomal-encapsulated Near-infrared Fluorophore as a Tool for In Vivo Optical Imaging

article

Production and Targeting of Monovalent Quantum Dots

article

Studying the Stoichiometry of Epidermal Growth Factor Receptor in Intact Cells using Correlative Microscopy

article

Identification of Metal Oxide Nanoparticles in Histological Samples by Enhanced Darkfield Microscopy and Hyperspectral Mapping

article

Surface Functionalization of Hepatitis E Virus Nanoparticles Using Chemical Conjugation Methods

article

Light-sheet Fluorescence Microscopy for the Study of the Murine Heart

article

Correlative Light Electron Microscopy (CLEM) for Tracking and Imaging Viral Protein Associated Structures in Cryo-immobilized Cells

article

Immobilization of Live Caenorhabditis elegans Individuals Using an Ultra-thin Polydimethylsiloxane Microfluidic Chip with Water Retention

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved