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Ultrastructural Localization of Endogenous LC3 by On-Section Correlative Light-Electron Microscopy

DOI :

10.3791/65067-v

11:53 min

March 31st, 2023

March 31st, 2023

491 Views

1Center for Molecular Medicine-Cell Biology, University Medical Center Utrecht, Utrecht University

Here, we present a protocol for optimized on-section correlative light-electron microscopy based on endogenous, fluorescent labeling as a tool to investigate the localization of rare proteins in relation to cellular ultrastructure. The power of this approach is demonstrated by ultrastructural localization of endogenous LC3 in starved cells without Bafilomycin treatment.

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Biology

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