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Visualizing Subcellular Localization of a Protein in the Heart Using Quantum Dots-Mediated Immuno-Labeling Followed by Transmission Electron Microscopy

DOI :

10.3791/64085-v

8:13 min

September 16th, 2022

September 16th, 2022

2,095 Views

1Department of Pathology and Translational Pathobiology, Louisiana State University Health Sciences Center-Shreveport, 2Department of Molecular and Cellular Physiology, Louisiana State University Health Sciences Center-Shreveport

The present protocol describes a method of immuno-labeling a protein in the heart tissue sections using quantum dots. This technique provides a useful tool to visualize any protein's subcellular localization and expression at the ultrastructural level.

Tags

Subcellular Localization

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