September 16th, 2022
•The present protocol describes a method of immuno-labeling a protein in the heart tissue sections using quantum dots. This technique provides a useful tool to visualize any protein's subcellular localization and expression at the ultrastructural level.
Tags
Related Videos
Visualizing the Beating Heart in Drosophila
Preparation of embryos for Electron Microscopy of the Drosophila embryonic heart tube
Visualizing RNA Localization in Xenopus Oocytes
Detection of Nitric Oxide and Superoxide Radical Anion by Electron Paramagnetic Resonance Spectroscopy from Cells using Spin Traps
Nano-fEM: Protein Localization Using Photo-activated Localization Microscopy and Electron Microscopy
Plunge Freezing: A Tool for the Ultrastructural and Immunolocalization Studies of Suspension Cells in Transmission Electron Microscopy
Sample Preparation and Imaging of Exosomes by Transmission Electron Microscopy
Miniaturized Sample Preparation for Transmission Electron Microscopy
Detecting Protein Subcellular Localization by Green Fluorescence Protein Tagging and 4',6-Diamidino-2-phenylindole Staining in Caenorhabditis elegans
Biological Sample Preparation by High-pressure Freezing, Microwave-assisted Contrast Enhancement, and Minimal Resin Embedding for Volume Imaging
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved