Visualizing Subcellular Localization of a Protein in the Heart Using Quantum Dots-Mediated Immuno-Labeling Followed by Transmission Electron Microscopy

2.7K Views

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08:13 min

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September 16th, 2022

DOI :

10.3791/64085-v

September 16th, 2022

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Richa Aishwarya¹, Chowdhury S. Abdullah¹, Naznin Sultana Remex², Sadia Nitu¹, Brandon Hartman¹, Judy King¹, Md. Shenuarin Bhuiyan¹^,²

¹Department of Pathology and Translational Pathobiology, Louisiana State University Health Sciences Center-Shreveport, ²Department of Molecular and Cellular Physiology, Louisiana State University Health Sciences Center-Shreveport

Summary

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Subcellular Localization

Protein Function

Quantum Dots

Immuno labeling

Transmission Electron Microscopy

Photobleaching Resistance

Electron Density

Fixation Process

Sodium Cacodylate Buffer

Tissue Penetration

Glutaraldehyde Fixation

Osmium Tetroxide Solution

Micrometers Scale Dissection

Heart Tissue Preparation

Reagent Optimization

Chapters in this video

0:05

Introduction

1:16

Animal Preparation

2:39

Heart Tissue Processing and Embedding

3:47

Tissue Sectioning Using an Ultramicrotome and Ultrathin Heart Section Staining

6:19

Transmission Electron Microscopy Imaging

6:48

Results: Visualization of Sigmar1 Using Quantum Dots

7:22

Conclusion

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