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AFM and Microrheology in the Zebrafish Embryo Yolk Cell

DOI :

10.3791/56224-v

November 29th, 2017

November 29th, 2017

7,872 Views

1Instituto de Biología Molecular de Barcelona, Consejo Superior de Investigaciones Científicas, 2Institute for Bioengineering of Catalonia, Universitat de Barcelona and CIBER Enfermedades Respiratorias

The lack of tools to measure material properties and tensional parameters in vivo prevents validating their roles during development. We employed atomic force microscopy (AFM) and nanoparticle-tracking to quantify mechanical features on the intact zebrafish embryo yolk cell during epiboly. These methods are reliable and widely applicable avoiding intrusive interventions.

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Keywords AFM

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