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Affinity Purification of Chloroplast Translocon Protein Complexes Using the TAP Tag

DOI :

10.3791/58532-v

7:01 min

November 1st, 2018

November 1st, 2018

9,717 Views

1Laboratory of Plant Physiology, University of Neuchatel, 2Institut fur Biochemie und Biotechnologie, Martin-Luther-Universitat Halle-Wittenberg

We here present a proven and tested protocol for the purification of chloroplast protein import complexes (TOC-TIC complex) using the TAP-tag. The one-step affinity-isolation protocol can potentially be applied to any protein and be used to identify new interaction partners by mass spectrometry.

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Chloroplast Translocon Protein Complexes

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