Flow-sorting and Exome Sequencing of the Reed-Sternberg Cells of Classical Hodgkin Lymphoma
9.9K Views
•
08:53 min
•
June 10th, 2017
DOI :
June 10th, 2017
•Jonathan B. Reichel1, Jason McCormick2, Jonathan R. Fromm3, Olivier Elemento4, Ethel Cesarman5, Mikhail Roshal6
1Innovation Laboratory, Center for Molecular Oncology, Memorial Sloan Kettering Cancer Center, 2Flow-Sorting Core Facility, Weill Cornell Medical College, 3Department of Laboratory Medicine, University of Washington, 4Department of Physiology and Biophysics, Institute for Computational Biomedicine, Weill Cornell Medical College, 5Department of Pathology and Laboratory Medicine, Weill Cornell Medical College, 6Department of Laboratory Medicine, Memorial Sloan Kettering Cancer Center
Chapters in this video
0:05
Title
1:19
Cell Suspension Preparation for Sorting
3:00
Compensation Control Setup
4:53
Hodgkin Reed-Sternberg-, B-, and T-cell Isolation
6:07
Results: Representative DNA Library and Copy Number Gains and Losses Analyses
8:02
Conclusion
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