Separation of Immune Cell Subpopulations in Peripheral Blood Samples from Children with Infectious Mononucleosis
2.3K Views
•
08:44 min
•
September 7th, 2022
DOI :
September 7th, 2022
•Linlin Zhang1,2, Mengjia Liu1,2, Meng Zhang1,2, Junhong Ai1,2, Jiao Tian1,2, Ran Wang1,2, Zhengde Xie1,2
1Beijing Key Laboratory of Pediatric Respiratory Infectious Diseases, Key Laboratory of Major Diseases in Children, Ministry of Education, National Clinical Research Center for Respiratory Diseases, Laboratory of Infection and Virology, Beijing Pediatric Research Institute, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, 2Research Unit of Critical Infection in Children, 2019RU016, Chinese Academy of Medical Sciences
Chapters in this video
0:05
Introduction
0:36
Isolation of CD14+ Monocytes from PBMCs Using CD14 Microbeads
2:38
Fluorescently Labeled Antibody Staining
3:52
Flow Cytometry Parameter Setting
4:32
Gating
6:18
Cell Sorting and Collection via Flow Cytometry
7:00
Results: Comparison of Immune Cell Subpopulations from Patients with Infectious Mononucleosis, Healthy Epstein Barr Virus Carriers, and Uninfected Samples
8:14
Conclusion
Related Videos
07:12
Neutrophil Isolation Protocol
102.0K Views
14:06
Isolation of Precursor B-cell Subsets from Umbilical Cord Blood
18.0K Views
09:08
Isolating Human Peripheral Blood Mononuclear Cells and CD4+ T cells from Sézary Syndrome Patients for Transcriptomic Profiling
5.3K Views
08:09
Efficient Isolation Protocol for B and T Lymphocytes from Human Palatine Tonsils
15.8K Views
08:26
The Isolation, Differentiation, and Quantification of Human Antibody-secreting B Cells from Blood: ELISpot as a Functional Readout of Humoral Immunity
15.1K Views
10:58
Discrimination of Seven Immune Cell Subsets by Two-fluorochrome Flow Cytometry
13.8K Views
06:23
Isolating Human Peripheral Blood Mononuclear Cells from Buffy Coats via High Throughput Immunomagnetic Bead Separation
2.8K Views
01:57
Isolation of Monocytes from Whole Blood by Immunomagnetic Negative Selection
944 Views
01:47
Isolation of Peripheral Blood Mononuclear Cells from Human Buffy Coats
976 Views
01:48
Isolation and Purification of B Cells from Human Peripheral Blood
235 Views
Copyright © 2025 MyJoVE Corporation. All rights reserved