Adenofection: A Method for Studying the Role of Molecular Chaperones in Cellular Morphodynamics by Depletion-Rescue Experiments

7.6K Views

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12:34 min

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September 16th, 2016

DOI :

10.3791/54557-v

September 16th, 2016

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Margit Fuchs¹^,², Marie-Chloé Boulanger³^,⁴, Herman Lambert¹^,², Jacques Landry¹^,², Josée N. Lavoie¹^,²

¹Département de biologie moléculaire, biochimie médicale et pathologie, Faculté de médecine, Centre de recherche sur le cancer de l'Université Laval, ²Oncology, Centre de recherche du CHU de Québec, Université Laval, ³Laboratoire d'études moléculaires des valvulopathies (LEMV), Groupe de recherche en valvulopathies (GRV), Quebec Heart and Lung Institute/Research Center, ⁴Department of Surgery, Université Laval

Summary

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Keywords Adenofection

Molecular Chaperones

Cellular Morphodynamics

Depletion rescue Experiments

Chapters in this video

0:05

Title

0:49

Plating HeLa-RFP-H2B Cells

3:38

Adenovirus Transduction and Endogenous Protein Knockdown by siRNA Transfection in HeLa-RFP-H2B Cells Using CaPi Precipitates

7:46

Live Cell Imaging of Mitotic Cells and Data Analysis

9:36

Results: Adenofection Effectively Introduces Proteins of Interest without Affecting Mitosis

11:44

Conclusion

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